Difference between revisions of "Part:BBa K2433004:Design"
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===Design Notes===
 
===Design Notes===
−
The VirB1 promoter was designed by taking the promoter region of virB1 from the pTiC58 - a Ti plasmid from Agrobacterium Tumefaciens. The nucleotide at position __ was changed to a T on the coding strand to eliminate an XbaI site.  
+
The VirB1 promoter was designed by taking the promoter region of virB1 from the pTiC58 - a Ti plasmid from Agrobacterium Tumefaciens. The nucleotide at position 339 was changed to a T on the coding strand to eliminate an XbaI cutsite.  
  
 
===Source===
 
===Source===

Revision as of 06:13, 1 November 2017


VirB1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 118


Design Notes

The VirB1 promoter was designed by taking the promoter region of virB1 from the pTiC58 - a Ti plasmid from Agrobacterium Tumefaciens. The nucleotide at position 339 was changed to a T on the coding strand to eliminate an XbaI cutsite.

Source

Upstream regulatory region of virB1 from pTiC58.

References

NEED TO ADD