Difference between revisions of "Part:BBa K2272002"

Latest revision as of 05:35, 1 November 2017

HAO (hydroxylamine oxidoreductase)

This coding sequence in Nitrosomonas europaea, a nitrifying bacterium, codes for hydroxylamine oxidoreductase, which catalyzes the oxidation of hydroxylamine to nitrite. The P. denitrificans TAT signal peptide coding sequences are embedded in the HAO coding sequence to direct export the hydroxylamine oxidoreductase to the periplasm of the cell. For further characterization information, refer to our final device: https://parts.igem.org/wiki/index.php?title=Part:BBa_K2272020

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 339
Illegal XhoI site found at 1903
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 355
Illegal NgoMIV site found at 832
Illegal NgoMIV site found at 836
Illegal NgoMIV site found at 2604
Illegal NgoMIV site found at 2994
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 259
Illegal BsaI.rc site found at 307
Illegal BsaI.rc site found at 874
Illegal BsaI.rc site found at 3034

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2272002 short</partinfo>
-This coding sequence in Nitrosomonas europaea, a nitrifying bacterium, codes for hydroxylamine oxidoreductase, which catalyzes the oxidation of hydroxylamine to nitrite.
+This coding sequence in Nitrosomonas europaea, a nitrifying bacterium, codes for hydroxylamine oxidoreductase, which catalyzes the oxidation of hydroxylamine to nitrite. The P. denitrificans TAT signal peptide coding sequences are embedded in the HAO coding sequence to direct export the hydroxylamine oxidoreductase to the periplasm of the cell. For further characterization information, refer to our final device: https://parts.igem.org/wiki/index.php?title=Part:BBa_K2272020
 <!-- Add more about the biology of this part here