Difference between revisions of "Part:BBa K2384013"
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<partinfo>BBa_K2384013 short</partinfo> | <partinfo>BBa_K2384013 short</partinfo> | ||
| − | Xylose-inducible expression system: strong<b> P<i>xylA</i></b> promoter and repressor gene <b><i>xylR</i></b>; after xylose addition, the repressor is released from the P<i>xylA</i> that activates transcription initiation.(<a href="https://parts.igem.org/Part:BBa_K733002">BBa_K733002</a>) They have been designed for high expression in <i>B. megaterium</i> This part serves as a functional MT for heavy metal binding and conferred resistance toxic metal cadmium. GST as a fusion partenr is to enhance stability of expression.The original part was from Cornell(<a href="https://parts.igem.org/Part:BBa_K1460002">BBa_K1460002</a>). This year our parts were doing codon optimisation but without terminator codon. | + | <p>Xylose-inducible expression system: strong<b> P<i>xylA</i></b> promoter and repressor gene <b><i>xylR</i></b>; after xylose addition, the repressor is released from the P<i>xylA</i> that activates transcription initiation.(<a href="https://parts.igem.org/Part:BBa_K733002">BBa_K733002</a>) They have been designed for high expression in <i>B. megaterium</i> This part serves as a functional MT for heavy metal binding and conferred resistance toxic metal cadmium. GST as a fusion partenr is to enhance stability of expression.The original part was from Cornell(<a href="https://parts.igem.org/Part:BBa_K1460002">BBa_K1460002</a>). This year our parts were doing codon optimisation but without terminator codon.</p> |
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Revision as of 05:28, 1 November 2017
Repressor gene xylR+Strong PxylA promoter+RBS+GST-CRS5+His-tag
Xylose-inducible expression system: strong PxylA promoter and repressor gene xylR; after xylose addition, the repressor is released from the PxylA that activates transcription initiation.(<a href="https://parts.igem.org/Part:BBa_K733002">BBa_K733002</a>) They have been designed for high expression in B. megaterium This part serves as a functional MT for heavy metal binding and conferred resistance toxic metal cadmium. GST as a fusion partenr is to enhance stability of expression.The original part was from Cornell(<a href="https://parts.igem.org/Part:BBa_K1460002">BBa_K1460002</a>). This year our parts were doing codon optimisation but without terminator codon.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2159
Illegal XhoI site found at 847 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1571