Difference between revisions of "Part:BBa K354001"
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| − | The PlcR-PapR fusion peptide has been derived from the Bacillus cereus quorum signalling system. This group of Gram-positive bacteria employs the PlcR quorum system to initiate virulence factors under appropriate cell density conditions. Synthesized as a propeptide, this fusion molecule is exported from the cell via the secA pathway, cleaved by an extracellular protease and re-imported into the cell via an oligopeptide permease. Both the extracellular protease as well as olidopeptide permease are ubiquitous structures to the Gram-positive cell wall. A such, any gram-positive bacteria expressing the PlcR-PapR protein should be able to both process as well as import the activating form of the fusion peptide. | + | The PlcR-PapR fusion peptide has been derived from the <i>Bacillus cereus</i> quorum signalling system. This group of Gram-positive bacteria employs the PlcR quorum system to initiate virulence factors under appropriate cell density conditions. Synthesized as a propeptide, this fusion molecule is exported from the cell via the secA pathway, cleaved by an extracellular protease and re-imported into the cell via an oligopeptide permease. Both the extracellular protease as well as olidopeptide permease are ubiquitous structures to the Gram-positive cell wall. A such, any gram-positive bacteria expressing the PlcR-PapR protein should be able to both process as well as import the activating form of the fusion peptide. |
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Summary：In this improvement, we splited this part into two parts that can be used in cell-cell communication. | Summary：In this improvement, we splited this part into two parts that can be used in cell-cell communication. | ||
| − | From reference [1], the PlcR-PapR fusion peptide can activate gene expression without the PapR signal peptide. Because alteration of the export signal, this fusion peptide can not be exported and re-imported into the cell to activate the PlcR transcription factor. In our improvement, we provided two new parts. One is the PlcR protein (BBa_K2279002), the other is PapR signal peptide (BBa_K2279003). Combining these two new parts, we can build autoinduction system and Sender-Receiver Cells to enable cell-cell communication. | + | From reference [1], the PlcR-PapR fusion peptide can activate gene expression without the PapR signal peptide. Because alteration of the export signal, this fusion peptide can not be exported and re-imported into the cell to activate the PlcR transcription factor. In our improvement, we provided two new parts. One is the PlcR protein ([https://parts.igem.org/Part:BBa_K2279002 BBa_K2279002]), the other is PapR signal peptide ([https://parts.igem.org/Part:BBa_K2279003 BBa_K2279003]). Combining these two new parts, we can build autoinduction system and Sender-Receiver Cells to enable cell-cell communication. |
===Reference=== | ===Reference=== | ||
| − | [1] Pomerantsev A.P., Pomerantseva O.M. & Leppla S.H. (2004), A Spontaneous Translational Fusion of Bacillus cereus PlcR and PapR Activates Transcription of PlcR-Dependant Genes in Bacillus anthracis via Binding with a Specific Palindromic Sequence. Infection and Immunity 72:5814-5823. | + | [1] Pomerantsev A.P., Pomerantseva O.M. & Leppla S.H. (2004), A Spontaneous Translational Fusion of <i>Bacillus cereus</i> PlcR and PapR Activates Transcription of PlcR-Dependant Genes in Bacillus anthracis via Binding with a Specific Palindromic Sequence. Infection and Immunity 72:5814-5823. |
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Latest revision as of 05:11, 1 November 2017
PlcR-PapR - Gram+ve Quorum Peptide
The PlcR-PapR fusion peptide has been derived from the Bacillus cereus quorum signalling system. This group of Gram-positive bacteria employs the PlcR quorum system to initiate virulence factors under appropriate cell density conditions. Synthesized as a propeptide, this fusion molecule is exported from the cell via the secA pathway, cleaved by an extracellular protease and re-imported into the cell via an oligopeptide permease. Both the extracellular protease as well as olidopeptide permease are ubiquitous structures to the Gram-positive cell wall. A such, any gram-positive bacteria expressing the PlcR-PapR protein should be able to both process as well as import the activating form of the fusion peptide.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Improvement:TMMU-China 2017
Author：Yizhen Xu
Summary：In this improvement, we splited this part into two parts that can be used in cell-cell communication.
From reference [1], the PlcR-PapR fusion peptide can activate gene expression without the PapR signal peptide. Because alteration of the export signal, this fusion peptide can not be exported and re-imported into the cell to activate the PlcR transcription factor. In our improvement, we provided two new parts. One is the PlcR protein (BBa_K2279002), the other is PapR signal peptide (BBa_K2279003). Combining these two new parts, we can build autoinduction system and Sender-Receiver Cells to enable cell-cell communication.
Reference
[1] Pomerantsev A.P., Pomerantseva O.M. & Leppla S.H. (2004), A Spontaneous Translational Fusion of Bacillus cereus PlcR and PapR Activates Transcription of PlcR-Dependant Genes in Bacillus anthracis via Binding with a Specific Palindromic Sequence. Infection and Immunity 72:5814-5823.