Difference between revisions of "Part:BBa K2272020"
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*Due to glithes on the orginial part page (https://parts.igem.org/Part:BBa_S05411), this page was built to contain all information and description of the part without directly displaying the exact status of the actual submitted part. For ordering, status, and submission related inquries, please refer to the original part page, shown in the parenthesis above.
 
*Due to glithes on the orginial part page (https://parts.igem.org/Part:BBa_S05411), this page was built to contain all information and description of the part without directly displaying the exact status of the actual submitted part. For ordering, status, and submission related inquries, please refer to the original part page, shown in the parenthesis above.
  
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This is our final device.
 
  
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===Usage and Biology===
 
===Usage and Biology===
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[[File:Virginia_Device1_Steven.png|thumb|600px|center|Figure 1. This graph compares the efficacy of performing nitrification of our model organisms, Paracoccus Denitrificans and Nitrosomonas Europaea, versus our transformed chassis organism, Paracoccus Denitrificans with the HAO-CYT circuit. Efficacy was measured in change in the concentrations of ammonia over time, which showed efficiency of Nitrification in our Bioreactor.]]
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[[File:Virginia_Device2_Steven.png|thumb|600px|center|Figure 2. This graph compares the efficacy of producing nitrates, an end product of the nitrification process, between our model organisms, Paracoccus Denitrificans and Nitrosomonas Europaea, versus our transformed chassis organism, Paracoccus Denitrificans with the HAO-CYT circuit.]]
  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K2272020 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2272020 SequenceAndFeatures</partinfo>

Revision as of 05:08, 1 November 2017


pBAD-HAO-Cyt

  • Due to glithes on the orginial part page (https://parts.igem.org/Part:BBa_S05411), this page was built to contain all information and description of the part without directly displaying the exact status of the actual submitted part. For ordering, status, and submission related inquries, please refer to the original part page, shown in the parenthesis above.



Usage and Biology

Figure 1. This graph compares the efficacy of performing nitrification of our model organisms, Paracoccus Denitrificans and Nitrosomonas Europaea, versus our transformed chassis organism, Paracoccus Denitrificans with the HAO-CYT circuit. Efficacy was measured in change in the concentrations of ammonia over time, which showed efficiency of Nitrification in our Bioreactor.
Figure 2. This graph compares the efficacy of producing nitrates, an end product of the nitrification process, between our model organisms, Paracoccus Denitrificans and Nitrosomonas Europaea, versus our transformed chassis organism, Paracoccus Denitrificans with the HAO-CYT circuit.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 1556
    Illegal XhoI site found at 3120
    Illegal XhoI site found at 5329
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1572
    Illegal NgoMIV site found at 2049
    Illegal NgoMIV site found at 2053
    Illegal NgoMIV site found at 3821
    Illegal NgoMIV site found at 4211
    Illegal NgoMIV site found at 4478
    Illegal NgoMIV site found at 4526
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1476
    Illegal BsaI.rc site found at 1524
    Illegal BsaI.rc site found at 2091
    Illegal BsaI.rc site found at 4251
    Illegal BsaI.rc site found at 5928
    Illegal BsaI.rc site found at 6084
    Illegal SapI site found at 961