Difference between revisions of "Part:BBa K2272019"

 
 
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<partinfo>BBa_K2272019 short</partinfo>
 
<partinfo>BBa_K2272019 short</partinfo>
  
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Construction intermediate/device with pVHb promter.
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Device with VHb promter. For specific characterization of the HAO-Cyt coding sequence combination, refer to pBAD-HAO-Cyt(https://parts.igem.org/Part:BBa_K2272020). For characterization of pVHb, refer to https://parts.igem.org/Part:BBa_K2272000.
  
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
  
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<!-- -->
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An improved version of https://parts.igem.org/Part:BBa_K1067002 and https://parts.igem.org/Part:BBa_K1067004 by combining the two coding sequences, which were suppose to work hand in hand, together. The VHb promoter was inserted to regulate the nitrification circuit expression under certain hypoxic conditions usually found in Sewage tanks. The homolog developed by DTU-Denmark used what we believed to be an improper coding sequence for a one of the subunits, HAO-B. HAO-B is a putative protein, that at the time may not have been believed to be significant. However, after reviewing the sequence registered in the BLAST database, we believed that HAO-B played a fundamental role in the mechanism of HAO. Since the two parts work hand in hand to nitrify ammonium, we improved the parts from DTU-Denmark by combining the two parts, inserting condionally inducible promoters, and using signal peptides to transport the enzymes to their designated location in the cell.
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K2272019 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2272019 SequenceAndFeatures</partinfo>

Latest revision as of 04:41, 1 November 2017


pVHb-HAO-Cyt

Device with VHb promter. For specific characterization of the HAO-Cyt coding sequence combination, refer to pBAD-HAO-Cyt(https://parts.igem.org/Part:BBa_K2272020). For characterization of pVHb, refer to https://parts.igem.org/Part:BBa_K2272000.

Usage and Biology

An improved version of https://parts.igem.org/Part:BBa_K1067002 and https://parts.igem.org/Part:BBa_K1067004 by combining the two coding sequences, which were suppose to work hand in hand, together. The VHb promoter was inserted to regulate the nitrification circuit expression under certain hypoxic conditions usually found in Sewage tanks. The homolog developed by DTU-Denmark used what we believed to be an improper coding sequence for a one of the subunits, HAO-B. HAO-B is a putative protein, that at the time may not have been believed to be significant. However, after reviewing the sequence registered in the BLAST database, we believed that HAO-B played a fundamental role in the mechanism of HAO. Since the two parts work hand in hand to nitrify ammonium, we improved the parts from DTU-Denmark by combining the two parts, inserting condionally inducible promoters, and using signal peptides to transport the enzymes to their designated location in the cell.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 484
    Illegal XhoI site found at 2048
    Illegal XhoI site found at 4257
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 500
    Illegal NgoMIV site found at 977
    Illegal NgoMIV site found at 981
    Illegal NgoMIV site found at 2749
    Illegal NgoMIV site found at 3139
    Illegal NgoMIV site found at 3406
    Illegal NgoMIV site found at 3454
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 404
    Illegal BsaI.rc site found at 452
    Illegal BsaI.rc site found at 1019
    Illegal BsaI.rc site found at 3179
    Illegal BsaI.rc site found at 4856
    Illegal BsaI.rc site found at 5012