Difference between revisions of "Part:BBa K2194001:Design"

 
(→‎References)
 
(3 intermediate revisions by the same user not shown)
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
[update]
+
Primers used to PCR amplify <i>sbp</i> from the <i>Escherichia coli</i> MG1655 genome<sup>NOTE</sup>: <p>
−
 
+
5' <b>CGATCGTCTCACTCGA</b>ATGAACAAGTGGGGCGTAG3'</p><p>
 +
5' <b>GCATCGTCTCACTCTGCCA</b>TCAGCGTTTGCTGATCTG3'</p>
 +
<br>
  
 +
NOTE: The primer overhangs are <b>bolded</b>.
  
 
===Source===
 
===Source===
  
−
<i>Escherichia coli<i/> genomic sequence
+
We sourced the nucleotide sequence for <i>sbp</i> from the <i>Escherichia coli</i> MG1655 genome EcoCyc database (Accession ID EG10929). We used this sequence to design primers to PCR amplify the sequence from purified <i>Escherichia coli</i> MG1655 genome.
  
 
===References===
 
===References===
 +
Keseler et al. (2017), "EcoCyc: reflecting new knowledge about Escherichia coli K-12", Nucleic Acids Research 45:D543-50.

Latest revision as of 02:41, 1 November 2017


Sulfate Binding Protein (sbp) cds


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3
    Illegal BsaI.rc site found at 1006


Design Notes

Primers used to PCR amplify sbp from the Escherichia coli MG1655 genomeNOTE:

5' CGATCGTCTCACTCGAATGAACAAGTGGGGCGTAG3'

5' GCATCGTCTCACTCTGCCATCAGCGTTTGCTGATCTG3'


NOTE: The primer overhangs are bolded.

Source

We sourced the nucleotide sequence for sbp from the Escherichia coli MG1655 genome EcoCyc database (Accession ID EG10929). We used this sequence to design primers to PCR amplify the sequence from purified Escherichia coli MG1655 genome.

References

Keseler et al. (2017), "EcoCyc: reflecting new knowledge about Escherichia coli K-12", Nucleic Acids Research 45:D543-50.