Difference between revisions of "Part:BBa K2194001:Design"
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===Source=== | ===Source=== | ||
− | <i>Escherichia coli</i> | + | We sourced the nucleotide sequence for <i>sbp</i> from the <i>Escherichia coli</i> MG1655 genome EcoCyc database (Accession ID EG10929). We used this sequence to design primers to PCR amplify the sequence from purified <i>Escherichia coli</i> MG1655 genome. |
===References=== | ===References=== |
Revision as of 02:41, 1 November 2017
Sulfate Binding Protein (sbp) cds
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 3
Illegal BsaI.rc site found at 1006
Design Notes
Primers used to PCR amplify sbp from the Escherichia coli MG1655 genomeNOTE:5' CGATCGTCTCACTCGAATGAACAAGTGGGGCGTAG3'
5' GCATCGTCTCACTCTGCCATCAGCGTTTGCTGATCTG3'
NOTE: The primer overhangs are bolded.
Source
We sourced the nucleotide sequence for sbp from the Escherichia coli MG1655 genome EcoCyc database (Accession ID EG10929). We used this sequence to design primers to PCR amplify the sequence from purified Escherichia coli MG1655 genome.