Difference between revisions of "Part:BBa K2382003"
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===References=== | ===References=== | ||
pET-29a(+) 5.4kb - GenScript https://www.genscript.com/gsfiles/vector-map/bacteria/pET-29a.pdf | pET-29a(+) 5.4kb - GenScript https://www.genscript.com/gsfiles/vector-map/bacteria/pET-29a.pdf | ||
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| + | BioBrick Part <partinfo>BBa K525998</partinfo> | ||
Revision as of 01:37, 1 November 2017
T7 promoter & Lac operator and RBS from PET-29a
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
This part originates from pET-29 a (+) Vectors, and it is composed of T7 promoter, Lac operator, and RBS.
We tried to improve this promoter sequence at BBa_K525998 by adding an lac operator and making it RFC 10 compatible.
Characterization of the T7 promoter & Lac operator and RBS from PET-29a
References
pET-29a(+) 5.4kb - GenScript https://www.genscript.com/gsfiles/vector-map/bacteria/pET-29a.pdf
BioBrick Part No part name specified with partinfo tag.