Difference between revisions of "Part:BBa K2440014"

 
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<partinfo>BBa_K2440014 short</partinfo>
 
<partinfo>BBa_K2440014 short</partinfo>
  
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It is the target sequence of miR-195, a modularized DNA part from a set of chemically synthetic oligo DNA library.
  
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===Usage and Biology===
 
===Usage and Biology===
  
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MiRNA locker assembled by using this modularized DNA part was able to bind miR-195 in an Ago2 dependent manner, that is, knockdown of miR-195 was achieved by transfecting cells with miRNA locker.
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Hsa-miR-195 was firstly identified in lung, skin and kidney of mouse by cloning from mouse tissues.<sup>1</sup>
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MicroRNA-195 has been reported as a cancer-related microRNA in many human cancers. Researchers find that miR-195 directly targets the fatty acid synthase enzyme and negatively regulates the expression of fatty acid synthase and finally demonstrated that MiR-195 inhibits the proliferation and invasion of pancreatic cancer cells by targeting the fatty acid synthase.<sup>2</sup>
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===Sequence and Features===
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2440014 parameters</partinfo>
 
<partinfo>BBa_K2440014 parameters</partinfo>
 
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==Experimental Validation==
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This part is sequenced as correct after construction.
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==Reference==
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1.New microRNAs from mouse and human. Lagos-Quintana M, Rauhut R, Meyer J, Borkhardt A, Tuschl T RNA. 9:175-179(2003).
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2.MicroRNA-195 inhibits the proliferation and invasion of pancreatic cancer cells by targeting the fatty acid synthase/Wnt signaling pathway.Xu Z, Li C, Qu H, Li H, Gu Q, Xu J.

Latest revision as of 01:36, 1 November 2017


miR-195 target sequence

It is the target sequence of miR-195, a modularized DNA part from a set of chemically synthetic oligo DNA library.

Usage and Biology

MiRNA locker assembled by using this modularized DNA part was able to bind miR-195 in an Ago2 dependent manner, that is, knockdown of miR-195 was achieved by transfecting cells with miRNA locker.

Hsa-miR-195 was firstly identified in lung, skin and kidney of mouse by cloning from mouse tissues.1

MicroRNA-195 has been reported as a cancer-related microRNA in many human cancers. Researchers find that miR-195 directly targets the fatty acid synthase enzyme and negatively regulates the expression of fatty acid synthase and finally demonstrated that MiR-195 inhibits the proliferation and invasion of pancreatic cancer cells by targeting the fatty acid synthase.2

Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Experimental Validation

This part is sequenced as correct after construction.


Reference

1.New microRNAs from mouse and human. Lagos-Quintana M, Rauhut R, Meyer J, Borkhardt A, Tuschl T RNA. 9:175-179(2003).

2.MicroRNA-195 inhibits the proliferation and invasion of pancreatic cancer cells by targeting the fatty acid synthase/Wnt signaling pathway.Xu Z, Li C, Qu H, Li H, Gu Q, Xu J.