Difference between revisions of "Part:BBa K2440012"

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MiRNA locker assembled by using this modularized DNA part was able to bind miR-4470 in an Ago2 dependent manner, that is, knockdown of miR-4470 was achieved by transfecting cells with miRNA locker.
 
MiRNA locker assembled by using this modularized DNA part was able to bind miR-4470 in an Ago2 dependent manner, that is, knockdown of miR-4470 was achieved by transfecting cells with miRNA locker.
  
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Hsa-miR-4470 is one of the founding members of the miRNA family. It was first found in normal and malignant human B-cells1.And it is located in 8q12.3.Its function hasn't been researched yet.
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Hsa-miR-4470 is one of the founding members of the miRNA family. It was first found in normal and malignant human B-cells<sup>1</sup>.And it is located in 8q12.3.Its function hasn't been researched yet.
  
 
===Sequence and Features===
 
===Sequence and Features===
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This part is sequenced as correct after construction.
 
This part is sequenced as correct after construction.
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==Reference==
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1.Deep sequencing of the small RNA transcriptome of normal and malignant human B cells identifies hundreds of novel microRNAs. Jima DD, Zhang J, Jacobs C, Richards KL, Dunphy CH, Choi WW, Au WY, Srivastava G, Czader MB, Rizzieri DA, Lagoo AS, Lugar PL, Mann KP, Flowers CR, Bernal-Mizrachi L, Naresh KN, Evens AM, Gordon LI, Luftig M, Friedman DR, Weinberg JB, Thompson MA, Gill JI, Blood. 116:e118-e127(2010).

Latest revision as of 01:33, 1 November 2017


miR-4470 target sequence

It is the target sequence of miR-4470, a modularized DNA part from a set of chemically synthetic oligo DNA library.

Usage and Biology

MiRNA locker assembled by using this modularized DNA part was able to bind miR-4470 in an Ago2 dependent manner, that is, knockdown of miR-4470 was achieved by transfecting cells with miRNA locker.

Hsa-miR-4470 is one of the founding members of the miRNA family. It was first found in normal and malignant human B-cells1.And it is located in 8q12.3.Its function hasn't been researched yet.

Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Experimental Validation

This part is sequenced as correct after construction.


Reference

1.Deep sequencing of the small RNA transcriptome of normal and malignant human B cells identifies hundreds of novel microRNAs. Jima DD, Zhang J, Jacobs C, Richards KL, Dunphy CH, Choi WW, Au WY, Srivastava G, Czader MB, Rizzieri DA, Lagoo AS, Lugar PL, Mann KP, Flowers CR, Bernal-Mizrachi L, Naresh KN, Evens AM, Gordon LI, Luftig M, Friedman DR, Weinberg JB, Thompson MA, Gill JI, Blood. 116:e118-e127(2010).