Difference between revisions of "Part:BBa K2440035"
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MiRNA locker assembled by using this modularized DNA part was able to bind miR7491 in an Ago2 dependent manner, that is, knockdown of miR7491 was achieved by transfecting cells with miRNA locker. | MiRNA locker assembled by using this modularized DNA part was able to bind miR7491 in an Ago2 dependent manner, that is, knockdown of miR7491 was achieved by transfecting cells with miRNA locker. | ||
| − | Ghr-miR7491 was firstly found in cotton ovules. And its function is still unknown. | + | Ghr-miR7491 was firstly found in cotton ovules<sup>1</sup>. And its function is still unknown. |
===Sequence and Features=== | ===Sequence and Features=== | ||
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This part is sequenced as correct after construction. | This part is sequenced as correct after construction. | ||
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| + | ==Reference== | ||
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| + | 1.A comparative miRNAome analysis reveals seven fiber initiation-related and 36 novel miRNAs in developing cotton ovules.Wang ZM, Xue W, Dong CJ, Jin LG, Bian SM, Wang C, Wu XY, Liu JY Mol Plant. 5:889-900(2012). | ||
Latest revision as of 01:27, 1 November 2017
miR7491 target sequence
It is the target sequence of miR7491, a modularized DNA part from a set of chemically synthetic oligo DNA library.
Usage and Biology
MiRNA locker assembled by using this modularized DNA part was able to bind miR7491 in an Ago2 dependent manner, that is, knockdown of miR7491 was achieved by transfecting cells with miRNA locker.
Ghr-miR7491 was firstly found in cotton ovules1. And its function is still unknown.
Sequence and Features
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Experimental Validation
This part is sequenced as correct after construction.
Reference
1.A comparative miRNAome analysis reveals seven fiber initiation-related and 36 novel miRNAs in developing cotton ovules.Wang ZM, Xue W, Dong CJ, Jin LG, Bian SM, Wang C, Wu XY, Liu JY Mol Plant. 5:889-900(2012).