Difference between revisions of "Part:BBa K2440033"
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MiRNA locker assembled by using this modularized DNA part was able to bind miR3476 in an Ago2 dependent manner, that is, knockdown of miR3476 was achieved by transfecting cells with miRNA locker. | MiRNA locker assembled by using this modularized DNA part was able to bind miR3476 in an Ago2 dependent manner, that is, knockdown of miR3476 was achieved by transfecting cells with miRNA locker. | ||
| − | Ghr-miR3476-5p was firstly found in cotton TIGR Plant | + | Ghr-miR3476-5p was firstly found in cotton TIGR Plant<sup>1</sup>. And its function is still unknown. |
===Sequence and Features=== | ===Sequence and Features=== | ||
Revision as of 01:24, 1 November 2017
miR3476 target sequence
It is the target sequence of miR3476, a modularized DNA part from a set of chemically synthetic oligo DNA library.
Usage and Biology
MiRNA locker assembled by using this modularized DNA part was able to bind miR3476 in an Ago2 dependent manner, that is, knockdown of miR3476 was achieved by transfecting cells with miRNA locker.
Ghr-miR3476-5p was firstly found in cotton TIGR Plant1. And its function is still unknown.
Sequence and Features
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Experimental Validation
This part is sequenced as correct after construction.