Difference between revisions of "Part:BBa K2440028"
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MiRNA locker assembled by using this modularized DNA part was able to bind miR4249 in an Ago2 dependent manner, that is, knockdown of miR4249 was achieved by transfecting cells with miRNA locker. | MiRNA locker assembled by using this modularized DNA part was able to bind miR4249 in an Ago2 dependent manner, that is, knockdown of miR4249 was achieved by transfecting cells with miRNA locker. | ||
| − | Aly-miR4249 is a novel miRNA that was firstly found in Arabidopsis lyrata. And its function is still unknown. | + | Aly-miR4249 is a novel miRNA that was firstly found in Arabidopsis lyrata<sup>1</sup>. And its function is still unknown. |
===Sequence and Features=== | ===Sequence and Features=== | ||
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This part is sequenced as correct after construction. | This part is sequenced as correct after construction. | ||
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| + | ==Reference== | ||
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| + | 1.Arabidopsis lyrata small RNAs: transient MIRNA and small interfering RNA loci within the Arabidopsis genus. Ma Z, Coruh C, Axtell MJ Plant Cell. 22:1090-1103(2010). | ||
Latest revision as of 01:15, 1 November 2017
miR4249 target sequence
It is the target sequence of miR4249, a modularized DNA part from a set of chemically synthetic oligo DNA library.
Usage and Biology
MiRNA locker assembled by using this modularized DNA part was able to bind miR4249 in an Ago2 dependent manner, that is, knockdown of miR4249 was achieved by transfecting cells with miRNA locker.
Aly-miR4249 is a novel miRNA that was firstly found in Arabidopsis lyrata1. And its function is still unknown.
Sequence and Features
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Experimental Validation
This part is sequenced as correct after construction.
Reference
1.Arabidopsis lyrata small RNAs: transient MIRNA and small interfering RNA loci within the Arabidopsis genus. Ma Z, Coruh C, Axtell MJ Plant Cell. 22:1090-1103(2010).