Difference between revisions of "Part:BBa K2440025"
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MiRNA locker assembled by using this modularized DNA part was able to bind miR165a in an Ago2 dependent manner, that is, knockdown of miR165a was achieved by transfecting cells with miRNA locker. | MiRNA locker assembled by using this modularized DNA part was able to bind miR165a in an Ago2 dependent manner, that is, knockdown of miR165a was achieved by transfecting cells with miRNA locker. | ||
| − | Aly-miR165a-5p is a novel miRNA that was firstly found in Arabidopsis | + | Aly-miR165a-5p is a novel miRNA that was firstly found in Arabidopsis lyrata<sup>1</sup>. And its function is still unknown. |
===Sequence and Features=== | ===Sequence and Features=== | ||
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This part is sequenced as correct after construction. | This part is sequenced as correct after construction. | ||
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| + | ==Reference== | ||
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| + | 1.Arabidopsis lyrata small RNAs: transient MIRNA and small interfering RNA loci within the Arabidopsis genus. Ma Z, Coruh C, Axtell MJ Plant Cell. 22:1090-1103(2010). | ||
Latest revision as of 01:10, 1 November 2017
miR165a target sequence
It is the target sequence of miR165a, a modularized DNA part from a set of chemically synthetic oligo DNA library.
Usage and Biology
MiRNA locker assembled by using this modularized DNA part was able to bind miR165a in an Ago2 dependent manner, that is, knockdown of miR165a was achieved by transfecting cells with miRNA locker.
Aly-miR165a-5p is a novel miRNA that was firstly found in Arabidopsis lyrata1. And its function is still unknown.
Sequence and Features
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Experimental Validation
This part is sequenced as correct after construction.
Reference
1.Arabidopsis lyrata small RNAs: transient MIRNA and small interfering RNA loci within the Arabidopsis genus. Ma Z, Coruh C, Axtell MJ Plant Cell. 22:1090-1103(2010).