Difference between revisions of "Part:BBa K2213002"

 
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<partinfo>BBa_K2213002 short</partinfo>
 
<partinfo>BBa_K2213002 short</partinfo>
  
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The ethanolamine utilisation bacterial microcompartment (BMC) protein, EutLK, under control of the arabinose inducible promoter.
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The ethanolamine utilisation bacterial microcompartment (BMC) proteins, EutLK (from E. coli) under control of the arabinose inducible promoter. Contains RBS, terminators and all tetp components. EutL is tagged with His6. EutK is tagged with FLAG (see figure 1).
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<br>
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https://static.igem.org/mediawiki/parts/thumb/5/51/Lk_operon.png/800px-Lk_operon.png
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<!-- Add more about the biology of this part here
 
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Revision as of 01:09, 1 November 2017


araB_eutLK

The ethanolamine utilisation bacterial microcompartment (BMC) proteins, EutLK (from E. coli) under control of the arabinose inducible promoter. Contains RBS, terminators and all tetp components. EutL is tagged with His6. EutK is tagged with FLAG (see figure 1).
800px-Lk_operon.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1300
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1239
    Illegal BamHI site found at 2164
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1074
    Illegal AgeI site found at 1775
    Illegal AgeI site found at 1952
    Illegal AgeI site found at 2252
    Illegal AgeI site found at 2315
    Illegal AgeI site found at 2362
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1056