Difference between revisions of "Part:BBa K2440024"
(→‎Experimental Validation)
 
(2 intermediate revisions by the same user not shown)
Line 9: Line 9:
  
 
MiRNA locker assembled by using this modularized DNA part was able to bind miR817 in an Ago2 dependent manner, that is, knockdown of miR817 was achieved by transfecting cells with miRNA locker.
 
MiRNA locker assembled by using this modularized DNA part was able to bind miR817 in an Ago2 dependent manner, that is, knockdown of miR817 was achieved by transfecting cells with miRNA locker.
 +
 +
MiR817 was firstly found in Oryza sativa and its target positions are:Os02g12800(0) Os03g31180(0) Os02g07960(1.5) Os09g39910(2)Os03g29680(0),Os08g38620(2).Its predicted functions are related to Elongation factor, Diacylglycerol kinase, and ABC transporter.<sup>1</sup>
  
 
===Sequence and Features===
 
===Sequence and Features===
Line 26: Line 28:
  
 
This part is sequenced as correct after construction.
 
This part is sequenced as correct after construction.
 +
 +
 +
==Reference==
 +
 +
 +
1.Rice embryogenic calli express a unique set of microRNAs, suggesting regulatory roles of microRNAs in plant post-embryogenic development"Luo YC, Zhou H, Li Y, Chen JY, Yang JH, Chen YQ, Qu LH FEBS Lett. 580:5111-5116(2006).

Latest revision as of 01:08, 1 November 2017


miR817 target sequence

It is the target sequence of miR817, a modularized DNA part from a set of chemically synthetic oligo DNA library.

Usage and Biology

MiRNA locker assembled by using this modularized DNA part was able to bind miR817 in an Ago2 dependent manner, that is, knockdown of miR817 was achieved by transfecting cells with miRNA locker.

MiR817 was firstly found in Oryza sativa and its target positions are:Os02g12800(0) Os03g31180(0) Os02g07960(1.5) Os09g39910(2)Os03g29680(0),Os08g38620(2).Its predicted functions are related to Elongation factor, Diacylglycerol kinase, and ABC transporter.1

Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Experimental Validation

This part is sequenced as correct after construction.


Reference

1.Rice embryogenic calli express a unique set of microRNAs, suggesting regulatory roles of microRNAs in plant post-embryogenic development"Luo YC, Zhou H, Li Y, Chen JY, Yang JH, Chen YQ, Qu LH FEBS Lett. 580:5111-5116(2006).