Difference between revisions of "Part:BBa K2440022"
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MiRNA locker assembled by using this modularized DNA part was able to bind miR418 in an Ago2 dependent manner, that is, knockdown of miR418 was achieved by transfecting cells with miRNA locker. | MiRNA locker assembled by using this modularized DNA part was able to bind miR418 in an Ago2 dependent manner, that is, knockdown of miR418 was achieved by transfecting cells with miRNA locker. | ||
| − | Osa-miR418 was firstly found in Arabidopsis. It is located in Chr3, ds of at 3g18890(a) and we still does not find its homolog of known Arabidopsis miRNAs.1 | + | Osa-miR418 was firstly found in Arabidopsis. It is located in Chr3, ds of at 3g18890(a) and we still does not find its homolog of known Arabidopsis miRNAs.<sup>1</sup> |
===Sequence and Features=== | ===Sequence and Features=== | ||
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This part is sequenced as correct after construction. | This part is sequenced as correct after construction. | ||
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| + | ==Reference== | ||
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| + | 1.Prediction and identification of Arabidopsis thaliana microRNAs and their mRNA targets. Wang XJ, Reyes JL, Chua NH, Gaasterland T Genome Biol. 5:R65(2004). | ||
Latest revision as of 01:05, 1 November 2017
miR418 target sequence
It is the target sequence of miR418, a modularized DNA part from a set of chemically synthetic oligo DNA library.
Usage and Biology
MiRNA locker assembled by using this modularized DNA part was able to bind miR418 in an Ago2 dependent manner, that is, knockdown of miR418 was achieved by transfecting cells with miRNA locker.
Osa-miR418 was firstly found in Arabidopsis. It is located in Chr3, ds of at 3g18890(a) and we still does not find its homolog of known Arabidopsis miRNAs.1
Sequence and Features
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Experimental Validation
This part is sequenced as correct after construction.
Reference
1.Prediction and identification of Arabidopsis thaliana microRNAs and their mRNA targets. Wang XJ, Reyes JL, Chua NH, Gaasterland T Genome Biol. 5:R65(2004).