Difference between revisions of "Part:BBa K2440019"

 
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<partinfo>BBa_K2440019 short</partinfo>
 
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It is the target sequence of miR-2467, a modularized DNA part from a set of chemically synthetic oligo DNA library.
  
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===Usage and Biology===
 
===Usage and Biology===
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MiRNA locker assembled by using this modularized DNA part was able to bind miR-2467 in an Ago2 dependent manner, that is, knockdown of miR-2467 was achieved by transfecting cells with miRNA locker.
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Hsa-miR-2467-5p was firstly found in human breast tissue. It may be applied in tumor identity with other miRNAs.<sup>1</sup>
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By using ChIP-Seq, Genechip and miRNA-Seq techniques, hsa-miR-2467-5p was identified as a NF-κB target miRNA in TNFα-stimulated HeLa Cells.<sup>2</sup>
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===Sequence and Features===
  
 
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<partinfo>BBa_K2440019 parameters</partinfo>
 
<partinfo>BBa_K2440019 parameters</partinfo>
 
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==Experimental Validation==
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This part is sequenced as correct after construction.
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==Reference==
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1.Identification of new microRNAs in paired normal and tumor breast tissue suggests a dual role for the ERBB2/Her2 gene. Persson H, Kvist A, Rego N, Staaf J, Vallon-Christersson J, Luts L, Loman N, Jonsson G, Naya H, Hoglund M, Borg A, Rovira C Cancer Res. 71:78-86(2011).
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2.NF-κB target microRNAs and their target genes in TNFα-stimulated HeLa cells. Zhou F, Wang W, Xing Y, Wang T, Xu X, Wang J.

Latest revision as of 01:00, 1 November 2017


miR-2467 target sequence

It is the target sequence of miR-2467, a modularized DNA part from a set of chemically synthetic oligo DNA library.

Usage and Biology

MiRNA locker assembled by using this modularized DNA part was able to bind miR-2467 in an Ago2 dependent manner, that is, knockdown of miR-2467 was achieved by transfecting cells with miRNA locker.

Hsa-miR-2467-5p was firstly found in human breast tissue. It may be applied in tumor identity with other miRNAs.1

By using ChIP-Seq, Genechip and miRNA-Seq techniques, hsa-miR-2467-5p was identified as a NF-κB target miRNA in TNFα-stimulated HeLa Cells.2

Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Experimental Validation

This part is sequenced as correct after construction.


Reference

1.Identification of new microRNAs in paired normal and tumor breast tissue suggests a dual role for the ERBB2/Her2 gene. Persson H, Kvist A, Rego N, Staaf J, Vallon-Christersson J, Luts L, Loman N, Jonsson G, Naya H, Hoglund M, Borg A, Rovira C Cancer Res. 71:78-86(2011).

2.NF-κB target microRNAs and their target genes in TNFα-stimulated HeLa cells. Zhou F, Wang W, Xing Y, Wang T, Xu X, Wang J.