Difference between revisions of "Part:BBa K2440017"

(→‎Usage and Biology)
(→‎Experimental Validation)
 
(2 intermediate revisions by the same user not shown)
Line 9: Line 9:
 
MiRNA locker assembled by using this modularized DNA part was able to bind miR-875 in an Ago2 dependent manner, that is, knockdown of miR-875 was achieved by transfecting cells with miRNA locker.
 
MiRNA locker assembled by using this modularized DNA part was able to bind miR-875 in an Ago2 dependent manner, that is, knockdown of miR-875 was achieved by transfecting cells with miRNA locker.
  
−
Hsa-miR-875-3p was found in human cells, and it is located in rn3 chr7- 71209979-71209999.1
+
Hsa-miR-875-3p was found in human cells, and it is located in rn3 chr7- 71209979-71209999.<sup>1</sup>
  
−
In dilated cardiomyopathy patients, the expression levels of miR-618, miR-875-3p, miR-205, miR-194, miR-302a, miR-147, and miR-544 were found decreased. The expression levels of miR-518f and miR-454 were found increased in DCM patients. These miRNA level differences may provide the chance of using these miRNAs as new biomarkers.2
+
In dilated cardiomyopathy patients, the expression levels of miR-618, miR-875-3p, miR-205, miR-194, miR-302a, miR-147, and miR-544 were found decreased. And the expression levels of miR-518f and miR-454 were found increased in DCM patients. These miRNA level differences may provide the chance of using these miRNAs as new biomarkers.<sup>2</sup>
  
 
===Sequence and Features===
 
===Sequence and Features===
Line 29: Line 29:
  
 
This part is sequenced as correct after construction.
 
This part is sequenced as correct after construction.
 +
 +
 +
==Reference==
 +
 +
 +
1. A mammalian microRNA expression atlas based on small RNA library sequencing. Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M Cell. 129:1401-1414(2007).
 +
 +
2 Plasma microRNA profiling of children with idiopathic dilated cardiomyopathy. Enes Coşkun M, Kervancıoğlu M, Öztuzcu S, Yılmaz Coşkun F, Ergün S, Başpınar O, Kılınç M, Temel L, Coşkun MY.

Latest revision as of 00:55, 1 November 2017


miR-875 target sequence

It is the target sequence of miR-875, a modularized DNA part from a set of chemically synthetic oligo DNA library.

Usage and Biology

MiRNA locker assembled by using this modularized DNA part was able to bind miR-875 in an Ago2 dependent manner, that is, knockdown of miR-875 was achieved by transfecting cells with miRNA locker.

Hsa-miR-875-3p was found in human cells, and it is located in rn3 chr7- 71209979-71209999.1

In dilated cardiomyopathy patients, the expression levels of miR-618, miR-875-3p, miR-205, miR-194, miR-302a, miR-147, and miR-544 were found decreased. And the expression levels of miR-518f and miR-454 were found increased in DCM patients. These miRNA level differences may provide the chance of using these miRNAs as new biomarkers.2

Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Experimental Validation

This part is sequenced as correct after construction.


Reference

1. A mammalian microRNA expression atlas based on small RNA library sequencing. Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M Cell. 129:1401-1414(2007).

2 Plasma microRNA profiling of children with idiopathic dilated cardiomyopathy. Enes Coşkun M, Kervancıoğlu M, Öztuzcu S, Yılmaz Coşkun F, Ergün S, Başpınar O, Kılınç M, Temel L, Coşkun MY.