Difference between revisions of "Part:BBa K2440017"
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MiRNA locker assembled by using this modularized DNA part was able to bind miR-875 in an Ago2 dependent manner, that is, knockdown of miR-875 was achieved by transfecting cells with miRNA locker. | MiRNA locker assembled by using this modularized DNA part was able to bind miR-875 in an Ago2 dependent manner, that is, knockdown of miR-875 was achieved by transfecting cells with miRNA locker. | ||
| − | Hsa-miR-875-3p was found in human cells, and it is located in rn3 chr7- 71209979-71209999.1 | + | Hsa-miR-875-3p was found in human cells, and it is located in rn3 chr7- 71209979-71209999.<sup>1</sup> |
| − | In dilated cardiomyopathy patients, the expression levels of miR-618, miR-875-3p, miR-205, miR-194, miR-302a, miR-147, and miR-544 were found decreased. And the expression levels of miR-518f and miR-454 were found increased in DCM patients. These miRNA level differences may provide the chance of using these miRNAs as new biomarkers.2 | + | In dilated cardiomyopathy patients, the expression levels of miR-618, miR-875-3p, miR-205, miR-194, miR-302a, miR-147, and miR-544 were found decreased. And the expression levels of miR-518f and miR-454 were found increased in DCM patients. These miRNA level differences may provide the chance of using these miRNAs as new biomarkers.<sup>2</sup> |
===Sequence and Features=== | ===Sequence and Features=== | ||
Revision as of 00:54, 1 November 2017
miR-875 target sequence
It is the target sequence of miR-875, a modularized DNA part from a set of chemically synthetic oligo DNA library.
Usage and Biology
MiRNA locker assembled by using this modularized DNA part was able to bind miR-875 in an Ago2 dependent manner, that is, knockdown of miR-875 was achieved by transfecting cells with miRNA locker.
Hsa-miR-875-3p was found in human cells, and it is located in rn3 chr7- 71209979-71209999.1
In dilated cardiomyopathy patients, the expression levels of miR-618, miR-875-3p, miR-205, miR-194, miR-302a, miR-147, and miR-544 were found decreased. And the expression levels of miR-518f and miR-454 were found increased in DCM patients. These miRNA level differences may provide the chance of using these miRNAs as new biomarkers.2
Sequence and Features
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Experimental Validation
This part is sequenced as correct after construction.