Difference between revisions of "Part:BBa I732014"
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<partinfo>BBa_I732014 short</partinfo> | <partinfo>BBa_I732014 short</partinfo> | ||
| − | + | [aTc]->PoPs converter. When [http://openwetware.org/wiki/ATc aTc (anhydrotetracycline)] is present, the repression of Ptet ([[Part:BBa_R0040]]) by constitutively expressed tetR([[Part:BBa_C0040]])(regulated by constitutive promoter PlacIQ([[Part:BBa_I14032]])) is relieved. Thus the downstream sequence of Ptet could be transcribed. The tetR protein we use here is LVA-tagged, to reduce its half life for better fast kinetics. However, the data from our measurement shows that this converter is not fit for this project and is abandoned at last. | |
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Latest revision as of 05:17, 26 October 2007
[aTc] -> PoPS
[aTc]->PoPs converter. When [http://openwetware.org/wiki/ATc aTc (anhydrotetracycline)] is present, the repression of Ptet (Part:BBa_R0040) by constitutively expressed tetR(Part:BBa_C0040)(regulated by constitutive promoter PlacIQ(Part:BBa_I14032)) is relieved. Thus the downstream sequence of Ptet could be transcribed. The tetR protein we use here is LVA-tagged, to reduce its half life for better fast kinetics. However, the data from our measurement shows that this converter is not fit for this project and is abandoned at last.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]