Difference between revisions of "Part:BBa K2440015"
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<partinfo>BBa_K2440015 short</partinfo> | <partinfo>BBa_K2440015 short</partinfo> | ||
− | + | It is the target sequence of miR-654, a modularized DNA part from a set of chemically synthetic oligo DNA library. | |
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===Usage and Biology=== | ===Usage and Biology=== | ||
+ | MiRNA locker assembled by using this modularized DNA part was able to bind miR-654 in an Ago2 dependent manner, that is, knockdown of miR-654 was achieved by transfecting cells with miRNA locker. | ||
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+ | Hsa-miR-654-5p was firstly found in human colorectal cells by miRNA serial analysis of gene expression. And its function is still unknown.<sup>1</sup> | ||
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+ | ===Sequence and Features=== | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
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<partinfo>BBa_K2440015 parameters</partinfo> | <partinfo>BBa_K2440015 parameters</partinfo> | ||
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+ | ==Experimental Validation== | ||
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+ | This part is sequenced as correct after construction. | ||
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+ | ===Reference=== | ||
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+ | 1.The colorectal microRNAome. Cummins JM, He Y, Leary RJ, Pagliarini R, Diaz LA Jr, Sjoblom T, Barad O, Bentwich Z, Szafranska AE, Labourier E, Raymond CK, Roberts BS, Juhl H, Kinzler KW, Vogelstein B, Velculescu VE Proc Natl Acad Sci U S A. 103:3687-3692(2006). |
Latest revision as of 00:44, 1 November 2017
miR-654 target sequence
It is the target sequence of miR-654, a modularized DNA part from a set of chemically synthetic oligo DNA library.
Usage and Biology
MiRNA locker assembled by using this modularized DNA part was able to bind miR-654 in an Ago2 dependent manner, that is, knockdown of miR-654 was achieved by transfecting cells with miRNA locker.
Hsa-miR-654-5p was firstly found in human colorectal cells by miRNA serial analysis of gene expression. And its function is still unknown.1
Sequence and Features
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Experimental Validation
This part is sequenced as correct after construction.
Reference
1.The colorectal microRNAome. Cummins JM, He Y, Leary RJ, Pagliarini R, Diaz LA Jr, Sjoblom T, Barad O, Bentwich Z, Szafranska AE, Labourier E, Raymond CK, Roberts BS, Juhl H, Kinzler KW, Vogelstein B, Velculescu VE Proc Natl Acad Sci U S A. 103:3687-3692(2006).