Difference between revisions of "Part:BBa K2440013"

Revision as of 00:39, 1 November 2017

miR-16 target sequence

It is the target sequence of miR-16, a modularized DNA part from a set of chemically synthetic oligo DNA library.

Usage and Biology

MiRNA locker assembled by using this modularized DNA part was able to bind miR-16 in an Ago2 dependent manner, that is, knockdown of miR-16 was achieved by transfecting cells with miRNA locker.

MiR-16 was firstly detected in mouse kidney, adult zebrafish, and very weakly in frog ovary¹. Located at chromosome 13q1², it is encoded by the mir-15a/mir16-1 and the mir-15b/mir16-2polycistrons.And its relative cloning frequency varied greatly and was higher in tumor samples and cell lines compared to normal tissues³.

Reductions in expression of miR-15a and miR-16, let-7a, or miR-143 and miR-145 have been reported in chronic lymphocytic leukemia , lung cancer , and colorectal neoplasia , respectively⁴.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Experimental Validation

This part is sequenced as correct after construction.

@@ Line 9: / Line 9: @@
 MiRNA locker assembled by using this modularized DNA part was able to bind miR-16 in an Ago2 dependent manner, that is, knockdown of miR-16 was achieved by transfecting cells with miRNA locker.
-MiR-16 was firstly detected in mouse kidney, adult zebrafish, and very weakly in frog ovary1. Located at chromosome 13q12, it is encoded by the mir-15a/mir16-1 and the mir-15b/mir16-2polycistrons.And its relative cloning frequency varied greatly and was higher in tumor samples and cell lines compared to normal tissues3.
+MiR-16 was firstly detected in mouse kidney, adult zebrafish, and very weakly in frog ovary<sup>1</sup>. Located at chromosome 13q1<sup>2</sup>, it is encoded by the mir-15a/mir16-1 and the mir-15b/mir16-2polycistrons.And its relative cloning frequency varied greatly and was higher in tumor samples and cell lines compared to normal tissues<sup>3</sup>.
-Reductions in expression of miR-15a and miR-16, let-7a, or miR-143 and miR-145 have been reported in chronic lymphocytic leukemia , lung cancer , and colorectal neoplasia , respectively4.
+Reductions in expression of miR-15a and miR-16, let-7a, or miR-143 and miR-145 have been reported in chronic lymphocytic leukemia , lung cancer , and colorectal neoplasia , respectively<sup>4</sup>.
 ===Sequence and Features===