Difference between revisions of "Part:BBa K2213004"
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===Usage and Biology=== | ===Usage and Biology=== | ||
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| + | This part is a fusion of the PduD(1-20), cgPPK and mCherry followed by a hexahistidine tag. We intended the PduD(1-20) sequence to localise the cgPPK into a recombinant microcompartment and the mCherry would have allowed us to confirm its co-localisation in the microcompartment. | ||
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Revision as of 00:33, 1 November 2017
PduD(1-20)_cgPPK2_mCherry_His6
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Usage and Biology
This part is a fusion of the PduD(1-20), cgPPK and mCherry followed by a hexahistidine tag. We intended the PduD(1-20) sequence to localise the cgPPK into a recombinant microcompartment and the mCherry would have allowed us to confirm its co-localisation in the microcompartment.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 148
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1030
Illegal SapI.rc site found at 827