Difference between revisions of "Part:BBa K2443037"
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<partinfo>BBa_K2443037 short</partinfo> | <partinfo>BBa_K2443037 short</partinfo> | ||
| − | T7 RNA Polymerase is responsible for transcribing DNA into RNA and is under the regulation of the T7 promoter consensus sequence. It has been Codon optimized for use in <i>Escherichia coli</i> and contains a N-terminal | + | T7 RNA Polymerase is responsible for transcribing DNA into RNA and is under the regulation of the T7 promoter consensus sequence. It has been Codon optimized for use in <i>Escherichia coli</i> and contains a N-terminal hexahistidine tag. Under the regulation of T7 Promoter, RBS and double terminator. |
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| + | <h1> Improved Part </h1> | ||
| + | <p><b>Original part:</b>BBa_I2032 submitted by MIT 2006</p> | ||
| + | <p><b> Rational behind improvements:</b> BBa_I2032 encodes exclusively for the coding region of T7 RNA polymerase. In order to improve it and incorporate it into our Next vivo system we have Codon optimized for use in E. coli and attached a N-terminal hexahistidine tag with a serine glycine linker for easy purification. We have also optimized our T7 RNA polymerase to be overexpressed in BL21-Gold (DE3) cells by putting it under the control of a T7 promoter (BBa_I719005), RBS (BBa_B0034) and double terminator (BBa_B0015). </p> | ||
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Latest revision as of 00:12, 1 November 2017
T7 RNA Polymerase optimized for expression in E. coli
T7 RNA Polymerase is responsible for transcribing DNA into RNA and is under the regulation of the T7 promoter consensus sequence. It has been Codon optimized for use in Escherichia coli and contains a N-terminal hexahistidine tag. Under the regulation of T7 Promoter, RBS and double terminator.
Improved Part
Original part:BBa_I2032 submitted by MIT 2006
Rational behind improvements: BBa_I2032 encodes exclusively for the coding region of T7 RNA polymerase. In order to improve it and incorporate it into our Next vivo system we have Codon optimized for use in E. coli and attached a N-terminal hexahistidine tag with a serine glycine linker for easy purification. We have also optimized our T7 RNA polymerase to be overexpressed in BL21-Gold (DE3) cells by putting it under the control of a T7 promoter (BBa_I719005), RBS (BBa_B0034) and double terminator (BBa_B0015).
Sequence and Features</p>
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1719
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 275
Illegal NgoMIV site found at 1496
Illegal NgoMIV site found at 1943 - 1000COMPATIBLE WITH RFC[1000]