Difference between revisions of "Part:BBa K2253002"
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| + | ===Experimental Design=== | ||
The process of attempting to improve the stability of the original blue chromoprotein consisted of | The process of attempting to improve the stability of the original blue chromoprotein consisted of | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K2253002 SequenceAndFeatures</partinfo> | <partinfo>BBa_K2253002 SequenceAndFeatures</partinfo> | ||
| − | + | Through the codon optimization of this part, in comparison to the BBa_K592009 original chromoprotein, the sequenced codon optimized blue chromoprotein shows minimal mutations. | |
<!-- Uncomment this to enable Functional Parameter display | <!-- Uncomment this to enable Functional Parameter display | ||
Revision as of 00:04, 1 November 2017
Optimized Blue Chromoprotein
This is the original blue chromoprotein sequence (BBa_K592009) that has been codon optimized. The part has been made a basic part with the promoter and RBS sequence, BBa_K608002 in the pSB1C3 BioBrick backbone. Codon optimization allows it to function more efficiently in E.coli, therefore creating a lower metabolic burden on the cells that it is encoded in. When this sequence is put into a vector and transformed into a plasmid it shows a blue phenotype. Codon optimization also makes this part more stable, as the color is expected to be maintained for a longer period of time than the original, making it a good candidate to use as a biosensor for things like toxins, such as lead.
Experimental Design
The process of attempting to improve the stability of the original blue chromoprotein consisted of Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 694
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Through the codon optimization of this part, in comparison to the BBa_K592009 original chromoprotein, the sequenced codon optimized blue chromoprotein shows minimal mutations.