Difference between revisions of "Part:BBa K2443026"

 
 
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<partinfo>BBa_K2443026 short</partinfo>
 
<partinfo>BBa_K2443026 short</partinfo>
  
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Elongation factor G is a prokaryotic translocase and GTPase responsible for catalyzing and coordinating the movement of tRNA and mRNA through the ribosome. It has been Codon optimized for use in <i>Escherichia coli</i> and contains an N-terminal Histidine tag. Under the regulation of T7 Promoter, RBS and double terminator.  
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Elongation factor G is a prokaryotic translocase and GTPase responsible for catalyzing and coordinating the movement of tRNA and mRNA through the ribosome. It has been Codon optimized for use in <i>Escherichia coli</i> and contains an N-terminal hexahistidine tag. Under the regulation of T7 Promoter, RBS and double terminator.  
  
  

Latest revision as of 00:01, 1 November 2017


EF-G optimized for expression in E. coli

Elongation factor G is a prokaryotic translocase and GTPase responsible for catalyzing and coordinating the movement of tRNA and mRNA through the ribosome. It has been Codon optimized for use in Escherichia coli and contains an N-terminal hexahistidine tag. Under the regulation of T7 Promoter, RBS and double terminator.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 191
    Illegal AgeI site found at 629
    Illegal AgeI site found at 1268
    Illegal AgeI site found at 1330
    Illegal AgeI site found at 1918
    Illegal AgeI site found at 1973
    Illegal AgeI site found at 2039
  • 1000
    COMPATIBLE WITH RFC[1000]