Difference between revisions of "Part:BBa K2443018"

 
 
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<partinfo>BBa_K2443018 short</partinfo>
 
<partinfo>BBa_K2443018 short</partinfo>
  
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Threonine tRNA synthetase responsible for attaching threonine onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in <i>Escherichia coli</i>. Contains a C-terminal histidine tag with a serine glycine linker. Under the regulation of T7 Promoter, RBS and double terminator.  
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Threonine tRNA synthetase responsible for attaching threonine onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in <i>Escherichia coli</i>. Contains a C-terminal hexahistidine tag with a serine glycine linker. Under the regulation of T7 Promoter, RBS and double terminator.  
  
  

Latest revision as of 23:52, 31 October 2017


ThrRS optimized for expression in E. coli

Threonine tRNA synthetase responsible for attaching threonine onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in Escherichia coli. Contains a C-terminal hexahistidine tag with a serine glycine linker. Under the regulation of T7 Promoter, RBS and double terminator.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1933
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]