Difference between revisions of "Part:BBa K2443009"

Revision as of 23:26, 31 October 2017

HisRS optimized for expression in E. coli

Histidine tRNA synthetase responsible for attaching histidine onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in Escherichia coli. Contains a N-terminal hexahistidine tag with a serine glycine linker. Under the regulation of T7 Promoter, RBS and double terminator.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Revision as of 14:41, 18 October 2017 (view source) Graeme.glaister (Talk \| contribs) ← Older edit		Revision as of 23:26, 31 October 2017 (view source) Harlando (Talk \| contribs) Newer edit →
Line 3:		Line 3:
	<partinfo>BBa_K2443009 short</partinfo>		<partinfo>BBa_K2443009 short</partinfo>

−	Histidine tRNA synthetase responsible for attaching histidine onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in <i>Escherichia coli</i>. Contains a N-terminal ~~histidine~~ tag with a serine glycine linker. Under the regulation of T7 Promoter, RBS and double terminator.	+	Histidine tRNA synthetase responsible for attaching histidine onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in <i>Escherichia coli</i>. Contains a N-terminal hexahistidine tag with a serine glycine linker. Under the regulation of T7 Promoter, RBS and double terminator.