Difference between revisions of "Part:BBa K2443004"

Latest revision as of 23:20, 31 October 2017

CysRS optimized for expression in E. coli

Cysteine tRNA synthetase responsible for attaching cysteine onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in Escherichia coli. Contains a C-terminal hexahistidine tag with a serine glycine linker. Under the regulation of T7 Promoter, RBS and double terminator.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1042
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 908

Revision as of 17:59, 5 October 2017 (view source) Graeme.glaister (Talk \| contribs) ← Older edit		Latest revision as of 23:20, 31 October 2017 (view source) Harlando (Talk \| contribs)
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	<partinfo>BBa_K2443004 short</partinfo>		<partinfo>BBa_K2443004 short</partinfo>

−	Cysteine tRNA synthetase responsible for attaching cysteine onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in <i>Escherichia coli</i>. Contains a C-terminal ~~histidine~~ tag with a serine glycine linker. Under the regulation of T7 Promoter, RBS and double terminator.	+	Cysteine tRNA synthetase responsible for attaching cysteine onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in <i>Escherichia coli</i>. Contains a C-terminal hexahistidine tag with a serine glycine linker. Under the regulation of T7 Promoter, RBS and double terminator.