Difference between revisions of "Part:BBa K2443002"

Latest revision as of 23:10, 31 October 2017

AsnRS optimized for expression in E. coli

Asparagine tRNA synthetase responsible for attaching asparagine onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in Escherichia coli. Contains a C-terminal hexahistidine tag with a serine glycine linker. Under the regulation of T7 Promoter, RBS and double terminator.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NotI site found at 1059
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Revision as of 17:59, 5 October 2017 (view source) Graeme.glaister (Talk \| contribs) ← Older edit		Latest revision as of 23:10, 31 October 2017 (view source) Harlando (Talk \| contribs)
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	<partinfo>BBa_K2443002 short</partinfo>		<partinfo>BBa_K2443002 short</partinfo>

−	Asparagine tRNA synthetase responsible for attaching asparagine onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in <i>Escherichia coli</i>. Contains a C-terminal ~~histidine~~ tag with a serine glycine linker. Under the regulation of T7 Promoter, RBS and double terminator.	+	Asparagine tRNA synthetase responsible for attaching asparagine onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in <i>Escherichia coli</i>. Contains a C-terminal hexahistidine tag with a serine glycine linker. Under the regulation of T7 Promoter, RBS and double terminator.