Difference between revisions of "Part:BBa K2443000"
 
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Alanine tRNA synthetase responsible for attaching Alanine onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in Escherichia coli. Contains a N-terminal histidine tag with a serine glycine linker. Under the regulation of T7 Promoter (BBa_I719005), RBS (BBa_B0034) and double terminator (BBa_B0015).
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__NOTOC__
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<partinfo>BBa_K2443000 short</partinfo>
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Alanine tRNA synthetase responsible for attaching alanine onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in <i>Escherichia coli</i>. Contains a C-terminal hexahistidine tag with a serine glycine linker. Under the regulation of T7 Promoter, RBS and double terminator.  
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<!-- Add more about the biology of this part here
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2443000 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K2443000 parameters</partinfo>
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Latest revision as of 23:07, 31 October 2017


AlaRS optimized for expression in E. coli

Alanine tRNA synthetase responsible for attaching alanine onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in Escherichia coli. Contains a C-terminal hexahistidine tag with a serine glycine linker. Under the regulation of T7 Promoter, RBS and double terminator.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 513
    Illegal BglII site found at 720
    Illegal BamHI site found at 686
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1124
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2524
    Illegal BsaI site found at 2701
    Illegal BsaI.rc site found at 1073
    Illegal SapI site found at 1808