Difference between revisions of "Part:BBa K2382006:Design"
(→Design Notes) |
(→Design Notes) |
||
| (6 intermediate revisions by the same user not shown) | |||
| Line 7: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | For the purpose of purification through nickel-resin column, we added a 18-bp sequence which can code 6 histidines. In addition, we chose T7 promoter which contains lac operator to express this protein because it can be induced by IPTG. For the terminator, we chose BBa_B0015 because it was commonly used in E. coli. The gene design are shown in Fig. 1. | + | For the purpose of purification through nickel-resin column, we added a 18-bp sequence which can code 6 histidines. In addition, we chose T7 promoter which contains lac operator(BBa_K2382002) to express this protein because it can be induced by IPTG. For the terminator, we chose BBa_B0015 because it was commonly used in E. coli. The gene design are shown in Fig. 1. |
| + | |||
| + | |||
| + | |||
| + | [[File:T--CSMU_NCHU_Taiwan--Design2.png|500px|thumb|left|Fig. 1]] | ||
| + | |||
| + | <br style="clear: both" /> | ||
===Source=== | ===Source=== | ||
===References=== | ===References=== | ||
| + | 1. Taylor, M.C., et al., Identification and characterization of two families of F420H2‐dependent reductases from Mycobacteria that catalyse aflatoxin degradation. Molecular microbiology, 2010. 78(3): p. 561-575. | ||
Latest revision as of 23:00, 31 October 2017
T7 promoter + Thioredoxin-MSMEG_5998 fusion protein
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 456
Illegal XhoI site found at 462 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 901
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
For the purpose of purification through nickel-resin column, we added a 18-bp sequence which can code 6 histidines. In addition, we chose T7 promoter which contains lac operator(BBa_K2382002) to express this protein because it can be induced by IPTG. For the terminator, we chose BBa_B0015 because it was commonly used in E. coli. The gene design are shown in Fig. 1.
Source
References
1. Taylor, M.C., et al., Identification and characterization of two families of F420H2‐dependent reductases from Mycobacteria that catalyse aflatoxin degradation. Molecular microbiology, 2010. 78(3): p. 561-575.