Difference between revisions of "Part:BBa K2382006:Design"

 
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===Design Notes===
 
===Design Notes===
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acinia semper. Cras auctor, ex ac tincidunt mollis, ante ante fermentum mi, vitae porta purus lacus non turpis. Vestibulum ante ipsum primis in faucibus orci luctus et ultrices posuere cubilia Curae; Pellentesque in tempus elit. Pellentesque faucibus egestas molestie. Curabitur a leo a
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For the purpose of purification through nickel-resin column, we added a 18-bp sequence which can code 6 histidines. In addition, we chose T7 promoter which contains lac operator(BBa_K2382002) to express this protein because it can be induced by IPTG. For the terminator, we chose BBa_B0015 because it was commonly used in E. coli. The gene design are shown in Fig. 1.
  
  
  
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===Source===
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[[File:T--CSMU_NCHU_Taiwan--Design2.png|500px|thumb|left|Fig. 1]]
  
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acinia semper. Cras auctor, ex ac tincidunt mollis, ante ante fermentum mi, vitae porta purus lacus non turpis. Vestibulum ante ipsum primis in faucibus orci luctus et ultrices posuere cubilia Curae; Pellentesque in tempus elit. Pellentesque faucibus egestas molestie. Curabitur a leo a
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===Source===
  
 
===References===
 
===References===
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1. Taylor, M.C., et al., Identification and characterization of two families of F420H2‐dependent reductases from Mycobacteria that catalyse aflatoxin degradation. Molecular microbiology, 2010. 78(3): p. 561-575.

Latest revision as of 23:00, 31 October 2017


T7 promoter + Thioredoxin-MSMEG_5998 fusion protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 456
    Illegal XhoI site found at 462
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 901
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

For the purpose of purification through nickel-resin column, we added a 18-bp sequence which can code 6 histidines. In addition, we chose T7 promoter which contains lac operator(BBa_K2382002) to express this protein because it can be induced by IPTG. For the terminator, we chose BBa_B0015 because it was commonly used in E. coli. The gene design are shown in Fig. 1.


Fig. 1


Source

References

1. Taylor, M.C., et al., Identification and characterization of two families of F420H2‐dependent reductases from Mycobacteria that catalyse aflatoxin degradation. Molecular microbiology, 2010. 78(3): p. 561-575.