Difference between revisions of "Part:BBa K2455003"
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<partinfo>BBa_K2455003 short</partinfo> | <partinfo>BBa_K2455003 short</partinfo> | ||
− | This biobrick contains an AsiSI/Nb.BSMI USER Cassette with a N-terminal nona- | + | This biobrick contains an AsiSI/Nb.BSMI USER Cassette with a N-terminal nona-arginine (R9) Cell-penetrating peptide which gives proteins inserted into the cassette the ability to pass through plasma membranes. In addition the USER Cassette carries a N-terminal hexa-histidine tag which allows for easy purification. |
For our study we demonstrated that proteins inserted into the cassette can be purified, using the histidine-tag, | For our study we demonstrated that proteins inserted into the cassette can be purified, using the histidine-tag, |
Revision as of 21:55, 31 October 2017
Cell-Penetrating USER Cassette
This biobrick contains an AsiSI/Nb.BSMI USER Cassette with a N-terminal nona-arginine (R9) Cell-penetrating peptide which gives proteins inserted into the cassette the ability to pass through plasma membranes. In addition the USER Cassette carries a N-terminal hexa-histidine tag which allows for easy purification.
For our study we demonstrated that proteins inserted into the cassette can be purified, using the histidine-tag, and subsequently transferred into E. coli cells through the use of the R9 peptide.
In addition; previous studies have shown proteins associated with R9, either covalently or non-covalently, to be able to enter a variety of cell types (link!).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]