Difference between revisions of "Part:BBa K2273003:Design"

 
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===Design Notes===
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===Design===
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tba
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This part was generated with RFC10 Suffix and Prefix. It encodes for the <i>srfA</i> promoter that is part of the ComX quorum response of <i>B. subtilis</i> and was designed for the subproject "Communication" by the [http://2017.igem.org/Team:TU_Dresden/Project/Communication iGEM Team TU Dresden 2017 (EncaBcillus - It's a trap!)].<br>
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===Source===
 
===Source===
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The <i>comX</i> gene was amplified via PCR from the <i>B. subtilis</i> wild type W168 genome using the primers listed below.
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<br>
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<table>
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<tr>
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<td width="150">comX fwd RFC10</td>
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<td>gatcGAATTCGCGGCCGCTTCTAGAAGGAGGTCAAAAATGCAAGACCTAATTAACTAC</td>
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</tr>
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<tr>
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<td>comX rev RFC 10</td>
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<td>gatcCTGCAGCGGCCGCTACTAGTTTAATCACCCCATTGACGGG</td>
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</tr>
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</table>
  
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gDNA of <i>Bacillus subtilis</i>
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===References===
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Jung J, Yu KO, Ramzi AB, Choe SH, Kim SW, Han SO (2012) <i>Improvement of surfactin production in Bacillus subtilis using synthetic wastewater by overexpression of specific extracellular signaling peptides, comX and phrC.</i> Biotechnol Bioeng. 109(9):2349-56. doi: 10.1002/bit.24524. Epub 2012 Apr 24. [https://www.ncbi.nlm.nih.gov/pubmed/22511326 PubMed]
  
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===References===
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Hamoen LW, Venema G, Kuipers OP (2003) <i>Controlling competence in Bacillus subtilis: shared use of regulators.</i> Microbiology. 149(Pt 1):9-17 [https://www.ncbi.nlm.nih.gov/pubmed/?term=Controlling+competence+in+Bacillus+subtilis%3A+shared+use+of+regulators PubMed]<br><br>

Revision as of 21:16, 31 October 2017


ComX pheromone for competence development in Bacillus subtilis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design

This part was generated with RFC10 Suffix and Prefix. It encodes for the srfA promoter that is part of the ComX quorum response of B. subtilis and was designed for the subproject "Communication" by the [http://2017.igem.org/Team:TU_Dresden/Project/Communication iGEM Team TU Dresden 2017 (EncaBcillus - It's a trap!)].

Source

The comX gene was amplified via PCR from the B. subtilis wild type W168 genome using the primers listed below.

comX fwd RFC10 gatcGAATTCGCGGCCGCTTCTAGAAGGAGGTCAAAAATGCAAGACCTAATTAACTAC
comX rev RFC 10 gatcCTGCAGCGGCCGCTACTAGTTTAATCACCCCATTGACGGG

References

Jung J, Yu KO, Ramzi AB, Choe SH, Kim SW, Han SO (2012) Improvement of surfactin production in Bacillus subtilis using synthetic wastewater by overexpression of specific extracellular signaling peptides, comX and phrC. Biotechnol Bioeng. 109(9):2349-56. doi: 10.1002/bit.24524. Epub 2012 Apr 24. PubMed

Hamoen LW, Venema G, Kuipers OP (2003) Controlling competence in Bacillus subtilis: shared use of regulators. Microbiology. 149(Pt 1):9-17 PubMed