Difference between revisions of "Part:BBa K2235005"

 
 
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<partinfo>BBa_K2235005 short</partinfo>
 
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Usage and Biology  
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==Usage and Biology==
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The sialidase enzyme was expressed from Arthrobacter ureafaciens (EC 3.2.1.18). This enzyme hydrolyzes glycosidic linkages of terminal sialic acid residues in glycoproteins.  
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Sialidase hydrolyses glycosidic linkages of terminal sialic acid residues in glycoproteins. In other words, it removes the sialic acids from the ends of the mucin constructs. This will help our bacteria to interact with the mucins for further degradation of the saccharides. Additionally, the removal of the sialic acids results in an exposure of other sugar groups on the mucins to other enzymes. Thus, the removal of sialic acids may result in even further mucus degradation by bacteria using different enzymes.
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[[File:SiaComp_Mechanism.png|400px|thumb|left|Figure 1: Schematic representation of Sialidase enzyme reaction mechanism.]]
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More details to the characterisation of Sialidase enzyme can be found in composite part BBa_K2235009.
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The following parts are composed of sialidase enzyme:
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<br>BBa_K2235006 biobrick has the RBS functional unit attached to sialidase part (BBa_K2235005) that can be used to test on various promoters.
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<br>BBa_K2235011 is a conjugation of sialidase composite (BBa_K2235009) with a device that facilitates the secretion of the enzyme out of the E.coli bacterial cell.
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<br>BBa_K2235007 biobrick constitutes OmpR fused to sialidase enzyme with RBS (BBa_K2235006).
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Latest revision as of 19:48, 31 October 2017


Sialidase enzyme

Usage and Biology

Sialidase hydrolyses glycosidic linkages of terminal sialic acid residues in glycoproteins. In other words, it removes the sialic acids from the ends of the mucin constructs. This will help our bacteria to interact with the mucins for further degradation of the saccharides. Additionally, the removal of the sialic acids results in an exposure of other sugar groups on the mucins to other enzymes. Thus, the removal of sialic acids may result in even further mucus degradation by bacteria using different enzymes.

Figure 1: Schematic representation of Sialidase enzyme reaction mechanism.



More details to the characterisation of Sialidase enzyme can be found in composite part BBa_K2235009. The following parts are composed of sialidase enzyme:
BBa_K2235006 biobrick has the RBS functional unit attached to sialidase part (BBa_K2235005) that can be used to test on various promoters.
BBa_K2235011 is a conjugation of sialidase composite (BBa_K2235009) with a device that facilitates the secretion of the enzyme out of the E.coli bacterial cell.
BBa_K2235007 biobrick constitutes OmpR fused to sialidase enzyme with RBS (BBa_K2235006).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 55
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 502
    Illegal NgoMIV site found at 577
    Illegal NgoMIV site found at 667
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1047