Difference between revisions of "Part:BBa K2340002:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | The sequence complementary | + | The sequence complementary to the human ꞵ-actin had to be exclusively complementary to that. If complementary to other mRNA sequences, the Cas13 could target those as well |
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===Source=== | ===Source=== | ||
| − | Sequences for crRNA backbone were taken from | + | Sequences for crRNA backbone were taken from East-Selesky et al (2016). |
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The complementary sequence to human ꞵ-actin mRNA and the sequences of the human U6 promoter and terminator are taken from Genbank | The complementary sequence to human ꞵ-actin mRNA and the sequences of the human U6 promoter and terminator are taken from Genbank | ||
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===References=== | ===References=== | ||
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| + | East-Selesky, A. <i>et al.</i> (2016) Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection. <i>Nature</i> <b>538:</b> 270–273. | ||
Latest revision as of 18:51, 31 October 2017
Guide RNA construct of human ꞵ-actin (2)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 217
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 183
Illegal XhoI site found at 224 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence complementary to the human ꞵ-actin had to be exclusively complementary to that. If complementary to other mRNA sequences, the Cas13 could target those as well
Source
Sequences for crRNA backbone were taken from East-Selesky et al (2016).
The complementary sequence to human ꞵ-actin mRNA and the sequences of the human U6 promoter and terminator are taken from Genbank
References
East-Selesky, A. et al. (2016) Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection. Nature 538: 270–273.