Difference between revisions of "Part:BBa K2340007:Design"
 
(2 intermediate revisions by the same user not shown)
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
The sequence complementary tot the human RAB13 had to be exclusively complementary to that. If complementary to other mRNA sequences, the Cas13 could target those as well
+
The sequence complementary to the human Pkp4 had to be exclusively complementary to that. If complementary to other mRNA sequences, the Cas13 could target those as well
  
  
Line 17: Line 17:
  
  
−
The complementary sequence to human RAB13 mRNA and the sequences of the human U6 promoter and terminator are taken from Genbank
+
The complementary sequence to human Pkp4 mRNA and the sequences of the human U6 promoter and terminator are taken from Genbank
  
  

Latest revision as of 18:49, 31 October 2017


Guide RNA construct of human Pkp4 (1)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 217
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 183
    Illegal XhoI site found at 224
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence complementary to the human Pkp4 had to be exclusively complementary to that. If complementary to other mRNA sequences, the Cas13 could target those as well


Source

Sequences for crRNA backbone were taken from East-Selesky et al (2016).


The complementary sequence to human Pkp4 mRNA and the sequences of the human U6 promoter and terminator are taken from Genbank


References

East-Selesky, A. et al. (2016) Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection. Nature 538: 270–273.