Difference between revisions of "Part:BBa K2403002"
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This part contains the RRE (Rev Response Element) of HIV-1 along with a T7 promoter for RNA expression. Together with the HIV-1 Rev protein part (BBa_K2403000), it can be used to efficiently export target RNAs from the eukaryotic nucleus out into the cytoplasm. Details are summarized in '''[https://parts.igem.org/Part:BBa_K2403000 this link]'''. | This part contains the RRE (Rev Response Element) of HIV-1 along with a T7 promoter for RNA expression. Together with the HIV-1 Rev protein part (BBa_K2403000), it can be used to efficiently export target RNAs from the eukaryotic nucleus out into the cytoplasm. Details are summarized in '''[https://parts.igem.org/Part:BBa_K2403000 this link]'''. | ||
| − | Reference | + | ===Reference=== |
[1]https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4041468/pdf/gku304.pdf | [1]https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4041468/pdf/gku304.pdf | ||
Revision as of 17:59, 31 October 2017
T7 promoter>RRE(Rev Response Element)
You can transport high molecular mRNA into cytoplasm if you can use this parts BBa_2403000. Rev protein binds to RRE(Rev Response Element).
This part contains the RRE (Rev Response Element) of HIV-1 along with a T7 promoter for RNA expression. Together with the HIV-1 Rev protein part (BBa_K2403000), it can be used to efficiently export target RNAs from the eukaryotic nucleus out into the cytoplasm. Details are summarized in this link.
Reference
[1]https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4041468/pdf/gku304.pdf
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]