Difference between revisions of "Part:BBa K2403002"

 
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===Usage and Biology===
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This part contains the RRE (Rev Response Element) of HIV-1 along with a T7 promoter for RNA expression. Together with the HIV-1 Rev protein part (BBa_K2403000), it can be used to efficiently export target RNAs from the eukaryotic nucleus out into the cytoplasm. Details are summarized in this link.
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Reference
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[1]https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4041468/pdf/gku304.pdf
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Revision as of 17:54, 31 October 2017


T7 promoter>RRE(Rev Response Element)

You can transport high molecular mRNA into cytoplasm if you can use this parts BBa_2403000. Rev protein binds to RRE(Rev Response Element).


This part contains the RRE (Rev Response Element) of HIV-1 along with a T7 promoter for RNA expression. Together with the HIV-1 Rev protein part (BBa_K2403000), it can be used to efficiently export target RNAs from the eukaryotic nucleus out into the cytoplasm. Details are summarized in this link.

Reference [1]https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4041468/pdf/gku304.pdf


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]