Difference between revisions of "Part:BBa K2340003"

 
 
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Human ꞵ-actin guide RNA construct with a human U6 promoter and human U6 terminator.
 
Human ꞵ-actin guide RNA construct with a human U6 promoter and human U6 terminator.
  
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===Usage and Biology===
 
  
 
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<span class='h3bb'>Sequence and Features</span>
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<span class='h3bb'><b><font size="+1"> Sequence and Features </font></b></span>
 
<partinfo>BBa_K2340003 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2340003 SequenceAndFeatures</partinfo>
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<b><font size="+1"> Usage and Biology </font></b>
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This product will be translated by RNA polymerase III to form a guide RNA. This small RNA strand can be processed by the
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CRISPR dCas13a from <i>Leptotrichia buccalis</i> (LbuC2c2; [https://parts.igem.org/Part:BBa_K2340000 BBa_K2340000]) to form a CRISPR RNA (crRNA).
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The sequence that is complementary to Human &#42933;-actin is GAAAGCAATGCTATCACCTC. This is one of three Human &#42933;-actin guide RNA constructs we have constructed
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<b> DNA for this part has not been submitted as a sample to the registry due to time restraints. </b>
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Latest revision as of 17:24, 31 October 2017


Guide RNA construct of human ꞵ-actin (3)

Human ꞵ-actin guide RNA construct with a human U6 promoter and human U6 terminator.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 217
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 183
    Illegal XhoI site found at 224
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

This product will be translated by RNA polymerase III to form a guide RNA. This small RNA strand can be processed by the CRISPR dCas13a from Leptotrichia buccalis (LbuC2c2; BBa_K2340000) to form a CRISPR RNA (crRNA).

The sequence that is complementary to Human ꞵ-actin is GAAAGCAATGCTATCACCTC. This is one of three Human ꞵ-actin guide RNA constructs we have constructed


DNA for this part has not been submitted as a sample to the registry due to time restraints.