Difference between revisions of "Part:BBa K2336035:Design"

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===Design Notes===
 
===Design Notes===
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None.
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We amplificated the oprF and Sitag separately as the amount of gene synthesized by IDT is too little.Then,we linked these two genes and got the plasmid with these two genes. We did the point mutation in this part.
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===Source===
 
===Source===

Latest revision as of 17:20, 31 October 2017


oprF-GS-Sitag-FLAG


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 72
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 639
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3
    Illegal AgeI site found at 1527
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We amplificated the oprF and Sitag separately as the amount of gene synthesized by IDT is too little.Then,we linked these two genes and got the plasmid with these two genes. We did the point mutation in this part.

Source

Synthesized by IDT. And we fused 2 sequences(oprF and Sitag)

References