Difference between revisions of "Part:BBa K2365051"
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| − | TPI1 Yeast promoter;Triose-phosphateisomerase, triosephosphate isomerase promoter. Constitutive expression; come from Saccharomyces cerevisiae BY4742 genomic sequence . | + | TPI1 Yeast promoter;Triose-phosphateisomerase, triosephosphate isomerase promoter. Constitutive expression; come from Saccharomyces cerevisiae BY4742 genomic sequence .It plays an important role in glycolysis, is essential for the energy generation, which has been found in almost all organisms, including mammals, insects, fungi, plants and most bacteria. |
Revision as of 16:31, 31 October 2017
TPI1 promotor
TPI1 Yeast promoter;Triose-phosphateisomerase, triosephosphate isomerase promoter. Constitutive expression; come from Saccharomyces cerevisiae BY4742 genomic sequence .It plays an important role in glycolysis, is essential for the energy generation, which has been found in almost all organisms, including mammals, insects, fungi, plants and most bacteria.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 47
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 119
Nature promoter from Saccharomyces cerevisiae genome amplification obtained constitutive promoter, the original gene are associated with the metabolism of yeast. The other life from the genome of Cyc1 was amplified by overlap extension terminator promoter-cyc1t fusion fragments between elements of the same source on the arm pairing reserved standard restriction sites, even on the expression vector prs423 GFP (S65T), inserted into yeast sey6210 test 440-550nm fluorescence intensity.
