Difference between revisions of "Part:BBa K2213012"
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A 24 hour induction was DAPI stained to determine microcompartment formation and tag localisation.<br> | A 24 hour induction was DAPI stained to determine microcompartment formation and tag localisation.<br> | ||
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| − | https://static.igem.org/mediawiki/2017/ | + | https://static.igem.org/mediawiki/2017/4/4f/LowSMNLKDAPIcells.png<br> |
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As shown, there is a heterogeneous distribution of mCherry signal indicating successful tag localisation and by proxy successful expression of Eut subunits. | As shown, there is a heterogeneous distribution of mCherry signal indicating successful tag localisation and by proxy successful expression of Eut subunits. | ||
<br> | <br> | ||
Revision as of 16:24, 31 October 2017
LacUV5_EutS_Tet_EutMN
/
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1260
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 4125
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 3451
Function:
This part was expressed alongside EutLK-Low-PduD-mCherry-PPK (https://parts.igem.org/Part:BBa_K2213013) to form EutSMNLK+Low_PduD+PPK which was characterized as follows.
A 24 hour induction was DAPI stained to determine microcompartment formation and tag localisation.
As shown, there is a heterogeneous distribution of mCherry signal indicating successful tag localisation and by proxy successful expression of Eut subunits.