Difference between revisions of "Part:BBa K2259081"
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<partinfo>BBa_K2259081 short</partinfo> | <partinfo>BBa_K2259081 short</partinfo> | ||
| − | This is the updated version of iGEM base vector [[Part:BBa_I51020]] that has been withdrawn from database due to encoding a ccdB toxin. Compared to BBa_I51020 the ccdB toxin was switched out to blue and white selection for picking colonies with replaced origin of replication. [[Part:BBa_I51020]] page can still be | + | This is the updated version of iGEM base vector [[Part:BBa_I51020]] that has been withdrawn from the database due to encoding a ccdB toxin. Compared to BBa_I51020 the ccdB toxin was switched out to blue and white selection for picking colonies with the replaced origin of replication. [[Part:BBa_I51020]] page can still be used as a reliable source for this part's design and function. |
| Line 15: | Line 15: | ||
==Base vector 2.0 in SynORI framework== | ==Base vector 2.0 in SynORI framework== | ||
| − | Engineering an improved, functional base vector 2.0 is crucial for SynORI framework. | + | Engineering an improved, functional base vector 2.0 is crucial for the SynORI framework. |
Building a modular a synthetic origin of replication is easiest when working in biobrick region. But for synthetic ORI this is only possible if there is no other origin of replication in the rest of the vector. Base vector 2.0 provides this, as it has its pUC replicon in biobrick site. | Building a modular a synthetic origin of replication is easiest when working in biobrick region. But for synthetic ORI this is only possible if there is no other origin of replication in the rest of the vector. Base vector 2.0 provides this, as it has its pUC replicon in biobrick site. | ||
| − | One can then replace the pUC origin of replication to SynORI system parts. Once the modular SynORI system is built, it can be | + | One can then replace the pUC origin of replication to SynORI system parts. Once the modular SynORI system is built, it can be transferred to another plasmid location and biobricks are then free to use for any task. |
Revision as of 16:08, 31 October 2017
Base vector 2.0 - a backbone for SynORI multi-plasmid framework
This is the updated version of iGEM base vector Part:BBa_I51020 that has been withdrawn from the database due to encoding a ccdB toxin. Compared to BBa_I51020 the ccdB toxin was switched out to blue and white selection for picking colonies with the replaced origin of replication. Part:BBa_I51020 page can still be used as a reliable source for this part's design and function.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found at 968
Illegal suffix found at 2107 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 968
Illegal NheI site found at 745
Illegal NheI site found at 2283
Illegal SpeI site found at 2108
Illegal PstI site found at 2122
Illegal NotI site found at 974
Illegal NotI site found at 2115 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 968
Illegal BglII site found at 1451 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found at 968
Illegal suffix found at 2108 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found at 968
Plasmid lacks a suffix.
Illegal XbaI site found at 983
Illegal SpeI site found at 2108
Illegal PstI site found at 2122 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
About SynORI
SynORI is a framework for multi-plasmid systems created by Vilnius-Lithuania 2017 which enables quick and easy workflow with multiple plasmids, while also allowing to freely pick and modulate copy number for every unique plasmid group! Read more about [http://2017.igem.org/Team:Vilnius-Lithuania SynORI here]!
Base vector 2.0 in SynORI framework
Engineering an improved, functional base vector 2.0 is crucial for the SynORI framework.
Building a modular a synthetic origin of replication is easiest when working in biobrick region. But for synthetic ORI this is only possible if there is no other origin of replication in the rest of the vector. Base vector 2.0 provides this, as it has its pUC replicon in biobrick site.
One can then replace the pUC origin of replication to SynORI system parts. Once the modular SynORI system is built, it can be transferred to another plasmid location and biobricks are then free to use for any task.
See [http://2017.igem.org/Team:Vilnius-Lithuania Vilnius-Lithuania 2017 team wiki] for more insight about our synthetic origin of replication (SynORI).