Difference between revisions of "Part:BBa K2247001"

 
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<partinfo>BBa_K2247001 short</partinfo>
 
<partinfo>BBa_K2247001 short</partinfo>
  
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This part is a EGFP gene under the pGalS yeast promoter, used to test the inducible expression from pGalS promoter
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This part encodes a GFP gene ([https://parts.igem.org/Part:BBa_K895006 BBa_K895006]) under the pGalS yeast promoter, used to test the Gal4-dependent inducible expression from pGalS promoter (for detail, see [https://parts.igem.org/Part:BBa_K2247000 BBa_K2247000]).
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===Usage and Biology===
 
===Usage and Biology===
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See  [https://parts.igem.org/Part:BBa_K2247000 BBa_K2247000].
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This part is mainly used to test the function of the yeast pGalS promoter (BBa_K2247000). The expression of GFP can be induced by galactose, or yeast Gal4 protein.
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===Characterization===
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[[File:PGalS-GFP AD-BD.jpeg|left|300px]]  [[File:PGalS-GFP AD-BD-2.jpeg|center|300px]]
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=====Figure 1. Yeast GFP expression under the pGalS promoter upon Gal4 induction.=====
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This figure shows the Gal4-inducible expression of GFP from the pGalS promoter (BBa_K2247000). The yeast strain JDYY001 (endogenous Gal4 and Gal80 knockout) was transformed with plasmids encoding pGalS-GFP and Gal4. These representative images showed successful expression of GFP under pGalS induced by Gal4 protein, validating the function of pGalS promoter.
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=====Note=====
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This part is an improvement of the part [https://parts.igem.org/Part:BBa_K895006 BBa_K895006].
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Latest revision as of 15:30, 31 October 2017


pGalS-GFP

This part encodes a GFP gene (BBa_K895006) under the pGalS yeast promoter, used to test the Gal4-dependent inducible expression from pGalS promoter (for detail, see BBa_K2247000).


Usage and Biology

See BBa_K2247000.

This part is mainly used to test the function of the yeast pGalS promoter (BBa_K2247000). The expression of GFP can be induced by galactose, or yeast Gal4 protein.


Characterization

PGalS-GFP AD-BD.jpeg
PGalS-GFP AD-BD-2.jpeg


Figure 1. Yeast GFP expression under the pGalS promoter upon Gal4 induction.

This figure shows the Gal4-inducible expression of GFP from the pGalS promoter (BBa_K2247000). The yeast strain JDYY001 (endogenous Gal4 and Gal80 knockout) was transformed with plasmids encoding pGalS-GFP and Gal4. These representative images showed successful expression of GFP under pGalS induced by Gal4 protein, validating the function of pGalS promoter.


Note

This part is an improvement of the part BBa_K895006.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 453
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 54
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1130