Difference between revisions of "Part:BBa K2247001"

(→‎Figure 1. Yeast GFP expression under the pGalS promoter upon Gal4 induction.)
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This figure shows the Gal4-inducible expression of GFP from the pGalS promoter (BBa_K2247000). The yeast strain JDYY001 (endogenous Gal4 and Gal80 knockout) was transformed with plasmids encoding pGalS-GFP and Gal4. These representative images showed successful expression of GFP under pGalS induced by Gal4 protein, validating the function of pGalS promoter.
 
This figure shows the Gal4-inducible expression of GFP from the pGalS promoter (BBa_K2247000). The yeast strain JDYY001 (endogenous Gal4 and Gal80 knockout) was transformed with plasmids encoding pGalS-GFP and Gal4. These representative images showed successful expression of GFP under pGalS induced by Gal4 protein, validating the function of pGalS promoter.
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======Note=====
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This part is an improvement of the part [https://parts.igem.org/Part:BBa_K895006 BBa_K895006].
  
  

Revision as of 15:27, 31 October 2017


pGalS-GFP

This part encodes a GFP gene (BBa_K895006) under the pGalS yeast promoter, used to test the Gal4-dependent inducible expression from pGalS promoter (for detail, see BBa_K2247000).


Usage and Biology

See BBa_K2247000.

This part is mainly used to test the function of the yeast pGalS promoter (BBa_K2247000). The expression of GFP can be induced by galactose, or yeast Gal4 protein.


Characterization

PGalS-GFP AD-BD.jpeg
PGalS-GFP AD-BD-2.jpeg


Figure 1. Yeast GFP expression under the pGalS promoter upon Gal4 induction.

This figure shows the Gal4-inducible expression of GFP from the pGalS promoter (BBa_K2247000). The yeast strain JDYY001 (endogenous Gal4 and Gal80 knockout) was transformed with plasmids encoding pGalS-GFP and Gal4. These representative images showed successful expression of GFP under pGalS induced by Gal4 protein, validating the function of pGalS promoter.


=Note

This part is an improvement of the part BBa_K895006.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 453
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 54
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1130