Difference between revisions of "Part:BBa K2336035"
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===Usage and Biology=== | ===Usage and Biology=== | ||
<p>oprF is motif cell wall anchor domain protein. GS stands for GS linker. Sitag-FLAG is designed to express specially protein. The genetic engineering bacteria can produce the protein on its membrane. Then the bacteria can be absorbed by silica surface. So we can capture them. Figure 1 shows the circuit of it. | <p>oprF is motif cell wall anchor domain protein. GS stands for GS linker. Sitag-FLAG is designed to express specially protein. The genetic engineering bacteria can produce the protein on its membrane. Then the bacteria can be absorbed by silica surface. So we can capture them. Figure 1 shows the circuit of it. | ||
| − | [[File: | + | [[File: SITAG.png|center|thumb|400px|Figure 1: The circuit of oprF-GS-Sitag-FLAG.]]</p> |
As we have said, this part is | As we have said, this part is | ||
Revision as of 15:23, 31 October 2017
oprF-GS-Sitag-FLAG
The report part of our circuit. It can be expressed to bind silicon
Usage and Biology
oprF is motif cell wall anchor domain protein. GS stands for GS linker. Sitag-FLAG is designed to express specially protein. The genetic engineering bacteria can produce the protein on its membrane. Then the bacteria can be absorbed by silica surface. So we can capture them. Figure 1 shows the circuit of it.
File:SITAG.png
Figure 1: The circuit of oprF-GS-Sitag-FLAG.
As we have said, this part is
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 72
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 639
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 3
Illegal AgeI site found at 1527 - 1000COMPATIBLE WITH RFC[1000]